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rabbit anti fap  (Novus Biologicals)


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    Novus Biologicals rabbit anti fap
    Rabbit Anti Fap, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+fibroblast+activation+protein+fap+antibody/Fibroblast+Activation+Protein+alpha%2FFAP+Antibody+(JA56-11)/pm41776828-79-91-92
    Average 94 stars, based on 4 article reviews
    rabbit anti fap - by Bioz Stars, 2026-10
    94/100 stars

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    Staining:

    Article Title: Verification of the role of exosomal microRNA in colorectal tumorigenesis using human colorectal cancer cell lines
    Article Snippet: .. CAFs were stained with anti-fibroblast activation protein (FAP) antibody (#427819, mouse monoclonal IgG 1 , Novus Biologicals, Littleton, CO, USA) followed by fluorescein isothiocyanate (FITC)-conjugated anti-mouse IgG 1 antibody. .. Jurkat and THP-1 cells were incubated with FITC-conjugated anti-human CCR1 (5F10B29, mouse monoclonal), allophycocyanin (APC)-conjugated anti-human CCR2 (K036C2, mouse monoclonal), and APC-conjugated anti-human CCR3 (5E8, mouse monoclonal) antibodies (all from BioLegend, San Diego, CA, USA) for 30 min on ice.

    Article Title: The role of exosomal miRNA-125b derived from colon cancer-associated fibroblasts in skeletal muscle cachexia
    Article Snippet: Proteins were detected using SuperSignalTM West Atto Substrate (a38554; Thermo Fisher Scientific) and visualized using a Bio-Rad ChemiDoc Imaging System (Bio-Rad Laboratories, Hercules, CA, USA). .. CAFs were labeled with an anti-fibroblast activation protein (FAP) antibody (Mouse monoclonal IgG1, 427819; Novus Biologicals, Littleton, CO, USA) and subsequently stained with a fluorescein isothiocyanate-conjugated anti-mouse IgG1 secondary antibody. .. After staining, the cells were centrifuged at 400 × g for 5 min at room temperature, fixed with 1% paraformaldehyde in flow cytometry staining buffer (0.5% FBS in PBS), and analyzed using a NovoCyte flow cytometer (ACEA Biosciences, San Diego, CA, USA) with NovoExpress software.

    Activation Assay:

    Article Title: Verification of the role of exosomal microRNA in colorectal tumorigenesis using human colorectal cancer cell lines
    Article Snippet: .. CAFs were stained with anti-fibroblast activation protein (FAP) antibody (#427819, mouse monoclonal IgG 1 , Novus Biologicals, Littleton, CO, USA) followed by fluorescein isothiocyanate (FITC)-conjugated anti-mouse IgG 1 antibody. .. Jurkat and THP-1 cells were incubated with FITC-conjugated anti-human CCR1 (5F10B29, mouse monoclonal), allophycocyanin (APC)-conjugated anti-human CCR2 (K036C2, mouse monoclonal), and APC-conjugated anti-human CCR3 (5E8, mouse monoclonal) antibodies (all from BioLegend, San Diego, CA, USA) for 30 min on ice.

    Article Title: The role of exosomal miRNA-125b derived from colon cancer-associated fibroblasts in skeletal muscle cachexia
    Article Snippet: Proteins were detected using SuperSignalTM West Atto Substrate (a38554; Thermo Fisher Scientific) and visualized using a Bio-Rad ChemiDoc Imaging System (Bio-Rad Laboratories, Hercules, CA, USA). .. CAFs were labeled with an anti-fibroblast activation protein (FAP) antibody (Mouse monoclonal IgG1, 427819; Novus Biologicals, Littleton, CO, USA) and subsequently stained with a fluorescein isothiocyanate-conjugated anti-mouse IgG1 secondary antibody. .. After staining, the cells were centrifuged at 400 × g for 5 min at room temperature, fixed with 1% paraformaldehyde in flow cytometry staining buffer (0.5% FBS in PBS), and analyzed using a NovoCyte flow cytometer (ACEA Biosciences, San Diego, CA, USA) with NovoExpress software.

    Labeling:

    Article Title: The role of exosomal miRNA-125b derived from colon cancer-associated fibroblasts in skeletal muscle cachexia
    Article Snippet: Proteins were detected using SuperSignalTM West Atto Substrate (a38554; Thermo Fisher Scientific) and visualized using a Bio-Rad ChemiDoc Imaging System (Bio-Rad Laboratories, Hercules, CA, USA). .. CAFs were labeled with an anti-fibroblast activation protein (FAP) antibody (Mouse monoclonal IgG1, 427819; Novus Biologicals, Littleton, CO, USA) and subsequently stained with a fluorescein isothiocyanate-conjugated anti-mouse IgG1 secondary antibody. .. After staining, the cells were centrifuged at 400 × g for 5 min at room temperature, fixed with 1% paraformaldehyde in flow cytometry staining buffer (0.5% FBS in PBS), and analyzed using a NovoCyte flow cytometer (ACEA Biosciences, San Diego, CA, USA) with NovoExpress software.



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